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Effect Of Fluoride And Laser On Artificial Caries-Like Lesion Formation In Bovine Enamel

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Abstract

°á·Ð
¹ý¶ûÁúÇ¥¸é¿¡¼­ ºÒ¼ÒµµÆ÷ ¹× ·¹ÀÌÀúÁ¶»ç°¡ ¹ÌÄ¡´Â Ç׿ì½ÄÈ¿°ú¸¦ ºñ±³Çϱâ À§ÇÏ¿©, °ÇÀüÇÑ
ÇÑ¿ì ÁßÀýÄ¡ÀÇ ¹ý¶ûÁú ¼ø¸éÀ» ´ë»óÀ¸·Î Àΰø ¿ì½Äº´¼Ò¸¦ Çü¼ºÇÑ ÈÄ ºÒ¼ÒµµÆ÷ ¹× ·¹ÀÌÀúÁ¶»ç
¸¦ ´Üµ¶ ¶Ç´Â º´¿ëÇÔ¿¡ µû¶ó Àΰø¿ì½Ä±º, ºÒ¼ÒµµÆ÷±º, ·¹ÀÌÀúÁ¶»ç±º, ºÒ¼ÒµµÆ÷ ÈÄ ·¹ÀÌÀú Á¶
»ç±º ±×¸®°í ·¹ÀÌÀúÁ¶»ç ÈÄ ºÒ¼ÒµµÆ÷±º µîÀÇ ½ÇÇ豺À¸·Î ºÐ·ùÇÑ ÈÄ ´ëÁ¶±ºÀ¸·Î¼­ Á¤»ó ¹ý¶û
ÁúÀ» ÀÌ¿ëÇÏ¿´´Ù. ºÒ¼ÒµµÆ÷ ¹× ·¹ÀÌÀúÁ¶»çµÈ °¢ ±ºÀÇ ¹ý¶ûÁú Ç¥¸éÀÇ ³»»ê¼º Æò°¡¸¦ À§ÇÑ ¹ý
¶ûÁúÀÇ ¿ëÇصµÃøÁ¤, ¹Ì¼¼°æµµÃøÁ¤, ÁÖ»çÀüÀÚ Çö¹Ì°æ °üÂû°ú ÇÔ²² EPMA¸¦ ÅëÇÑ ¹ý¶ûÁú ³»
ºÎÀÇ Ä®½·, ÀÎ ±×¸®°í ºÒ¼Ò¿ø¼ÒÀÇ Á¤¼º ¹× Á¤·®ºÐ¼®À» ½ÃÇàÇÑ °á°ú, ´ÙÀ½ÀÇ °á·ÐÀ» ¾ò¾ú´Ù.
1. ¹ý¶ûÁúÀÇ ³»»ê¼ºÀ» Æò°¡Çϱâ À§ÇÑ °¢ ±ºÀÇ Ä®½·¿ëÇØ·®À» ÃøÁ¤ÇÑ °á°ú, »ê¿ë¾×ÀÇ ³ëÃâ ½Ã
°£ÀÌ Áõ°¡ÇÔ¿¡ µû¶ó Ä®½·¿ëÇØ·®µµ Áõ°¡µÇ¾úÀ¸¸ç, Á¤»ó¹ý¶ûÁú±º¿¡ ºñÇØ ¸ðµç ½ÇÇ豺¿¡¼­ Çö
ÀúÈ÷ °¨¼ÒµÈ ¼öÄ¡·Î¼­ ³»»ê¼ºÀ» ³ªÅ¸³Â´Ù(P<0.001). ¶ÇÇÑ ºÒ¼ÒµµÆ÷ ¹× ·¹ÀÌÀúÁ¶»ç¸¦ º´¿ëÇÑ
½ÇÇ豺Àº ·¹ÀÌÀúÁ¶»ç±º¿¡ ºñÇØ ³·Àº ¼öÄ¡·Î¼­ À¯ÀÇÇÑ Â÷À̸¦ º¸¿´À¸³ª(P<0.05) ºÒ¼ÒµµÆ÷±º
°ú´Â À¯»çÇÑ °á°ú¸¦ ³ªÅ¸³Â´Ù(P>0.05).
2. Á¤»ó¹ý¶ûÁú±º¿¡¼­ ÃøÁ¤µÈ ¹Ì¼¼°æµµ´Â 295.47¡¾16(KHN)·Î¼­ ÃÖ´ë°ªÀ» º¸¿´°í, ¿ì½Äº´¼ÒÇü
¼º ÈÄ ºÒ¼ÒµµÆ÷ ¹× ·¹ÀÌÀúÁ¶»ç¸¦ ´Üµ¶ ¶Ç´Â º´¿ëÇÑ ½ÇÇ豺Àº ¿ì½Äº´¼Ò±ºº¸´Ù ÇöÀúÈ÷ Áõ°¡µÈ
°á°ú¸¦ ³ªÅ¸³Â´Ù(P<0.001). ¶ÇÇÑ ºÒ¼ÒµµÆ÷±ºÀº ·¹ÀÌÀúÁ¶»ç±º°ú ºÒ¼ÒµµÆ÷ ÈÄ ·¹ÀÌÀúÁ¶»ç±ºº¸
´Ù ³ô¾ÒÀ¸³ª, (P<0.001) ·¹ÀÌÀúÁ¶»ç ÈÄ ºÒ¼ÒµµÆ÷±º°ú´Â À¯»çÇÏ¿´´Ù(P>0.05).
3. ¿ì½Äº´¼ÒÇü¼º ÈÄ ºÒ¼ÒµµÆ÷¸¦ ½ÃÇàÇÑ ¹ý¶ûÁú Ç¥¸éÀÇ ¹Ì¼¼ÇüÅ´ ŻȸÈçÀûÀº »ç¶óÁö°í ºÒ
¼ÒÈ­ÇÕ¹°·Î º¸ÀÌ´Â ¼ö¸¹Àº °ú¸³ÀÚµéÀÌ ºÎÂøµÇ¾úÀ¸¸ç, fpÀÌÀúÁ¶»ç½Ã´Â ÇÑÃþ °æÈ­µÈ ¹ý¶ûÁúÇ¥
¸é¿¡ ¹Ì¼¼±Õ¿­°ú ÀÛÀº ¼Ò°øµéÀÌ Á¸ÀçÇÏ´Â ±Õ¿­»ó(Cracker-like appearance)À» ³ªÅ¸³Â´Ù. ºÒ
¼ÒµµÆ÷ ÈÄ ·¹ÀÌÀúÁ¶»ç¸¦ ½ÃÇàÇÑ °æ¿ì ºÒ¼Ò°ú¸³ÀÚµéÀÌ ±ä¹ÐÇÏ°Ô ¹ý¶ûÁúÇ¥¸é¿¡ ºÎÂøµÇ¾úÀ¸³ª
±Õ¿­ºÎ¿¡ ħÅõµÈ ÈçÀûÀº ¾ø¾úÀ¸¸ç, ·¹ÀÌÀúÁ¶»ç ÈÄ ºÒ¼ÒµµÆ÷½Ã ´Ù¼öÀÇ ºÒ¼ÒÈ­ÇÕ¹°ÀÌ ¼Ò°ø ¹×
±Õ¿­ºÎÀ§·Î ħÅõµÈ ¾ç»óÀÌ °üÂûµÇ¾ú´Ù.
4. EPMA¸¦ ÅëÇÏ¿© ¹ý¶ûÁú³»ºÎÀÇ ºÒ¼ÒºÐÆ÷ ¾ç»óÀ» ºÐ¼®ÇÑ °á°ú, ºÒ¼ÒµµÆ÷ ¹× ºÒ¼ÒµµÆ÷ ÈÄ
·¹ÀÌÀúÁ¶»ç¸¦ ½ÃÇàÇÑ ½ÃÆí¿¡¼­´Â ¹ý¶ûÁú ÃÖÇ¥ÃþÀ¸·ÎºÎÅÍ 5¥ìmÀ̳»¿¡¼­ ÇöÀúÇÏ°Ô Áõ°¡µÇ´Ù
°¡ ±Þ°ÝÇÑ °¨¼Ò¾ç»óÀ» ³ªÅ¸³ÂÀ¸³ª, ·¹ÀÌÀúÁ¶»ç ÈÄ ºÒ¼ÒµµÆ÷¸¦ ½ÃÇàÇÑ °æ¿ì´Â Ç¥ÃþºÎ 10¥ìm
±îÁö Áõ°¡µÊÀ¸·Î¼­ ºÒ¼ÒħÅõ È¿°ú°¡ °¡Àå Å« °ÍÀ¸·Î °üÂûµÇ¾ú´Ù.
5. ºÒ¼Ò ¹× ·¹ÀÌÀú »ç¿ë¿¡ µû¸¥ ¹ý¶ûÁúÀÇ Ä®½·/ÀÎ ¹«°Ôºñ´Â l0¥ìm Á¶»çÁ¡¿¡¼­ °ÇÀü ¹× ¿ì½Ä
¹ý¶ûÁúº¸´Ù ´Ù¼Ò Áõ°¡µÇ¾úÀ¸³ª ±× ÀÌ»óÀÇ ±íÀÌ¿¡¼­´Â À¯»çÇÑ °á°ú¸¦ ³ªÅ¸³Â´Ù. ºÒ¼Ò³óµµ ºÐ
¼®½Ã l0¥ìm Á¶»çÁ¡À» Á¦¿ÜÇÑ ¸ðµç Á¶»çÁ¡¿¡¼­ ºÒ¼ÒµµÆ÷±ºÀÌ ºÒ¼ÒµµÆ÷ ¹× ·¹ÀÌÀúÁ¶»ç°¡ º´
¿ëµÈ ½ÇÇ豺º¸´Ù ³ô°Ô ÃøÁ¤µÇ¾úÀ¸¸ç(P<0.05), ºÒ¼ÒµµÆ÷ ¹× ·¹ÀÌÀú Á¶»ç º´¿ë±º »çÀÌ¿¡ À¯ÀÇ
ÇÑ Â÷ÀÌ´Â ³ªÅ¸³ªÁö ¾Ê¾Ò´Ù(P>0.05).
#ÃÊ·Ï#
The purpose of this study was to examine the morphological characteristics and
combined effects of fluoride application and laser irradiation on artificial caries-like lesion
formation in bovine enamel. Enamel specimens were divided into five experiment group
and placed in no-treatment(group C), APF application alone(group F), laser irradiation
one(group L), APF application before laser irradiation (group FL), and APF application
after laser irradiation(group LF) on artificial caries-like lesion. Sound enamel was used
as a control group. The ultrastructural changes and physical effects of the fluorided and
lased enamel has been investigated by using SEM, enamel solubility and microhardness
test as well as distributions of calcium, phosphorus and fluoride in infernal enamel by
using EPMA. The following results were obtained.
1. In the all experiment groups, the amounts of dissolved calcium of enamel surfaces
significantly decreased according to increasing exposure time of acid solution than
control group(P<0.001). Group L showed higher than that group FL and LF in 30 and
60min(P<0.05).
2. The microhardness values of enamel surface in the control group was highest than
that in the other experiment groups. Group F, L, FL and LF were significantly increased
than group C(P<0.001).
3. The enamel surface treated with APF produced deposits of numerous small globules
and lased enamel showed a cracker-like appearance with microcrack and small pore.
Numerous deposits were infiltrated in the fissured portion of enamel treated with APF
after laser irradiation.
4. In the case of APF application alone, the elevation of the fluoride profile can be seen
within 5¥ìm of the outermost layer and a similar profile observed in the specimen
treated with APF before laser irradiation. However, the specimen treated with APF after
laser Irradiation showed a large elevation within loom of the outermost layer of the
enamel.
5. The higher Ca/P ratios were observed in 10¥ìm depth of lased and fluorided enamel
when compared to the sound and carious enamel. The fluoride content decreased rapidly
with distance from enamel surface, in the group F, fluoride concentration was
significantly higher than that in the group C, L, FL, LF and control group according to
increasing enamel depth(P<0.05).

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